The Journal of Insect Science: Volume 1/Article 10/Table 1

Table 1 The effect of mosquito numbers and potential virus uptake on defensin expression by MRE/3'2J/ppA or ppC virus in mosquitoes exposed as larvae to infected C6/36 cells.

	MRE/3'2J/ppA			MRE/3'2J/ppC
	Larvae²	Pupae²	Adults²	Larvae	Pupae	Adults
(a) 200 larvae¹	6/22³	8/22	13/20	18/22	21/22	15/20
	(27%)	(36%)	(65%)	(82%)	(95%)	(75%)

(b) 300 larvae	21/22	22/22	16/20	0/22	1/22	7/20
	(95%)	(100%)	(80%)	(0%)	(0.5%)	(35%)

¹With each viral construct, group (a) represents 200 larvae fed on 2.7 x 10⁷ virus-infected C6/36 cells, and group (b) represents 300 larvae.
²Larvae and pupae samples were collected 5 and 8 days respectively after exposure to virus-infected cells; adult hemolymph was collected 14 days after exposure (day 3 after eclosion).
³Values are numbers of defensin positive samples over total number of samples tested. Within treatment groups (different larvae numbers), comparisons of numbers of defensin positive samples were significantly different between groups (a) and (b) in larvae and pupae samples (P< 0.001), but were not significantly different in the adult samples (P = 0.423 for MRE/3'2J/ppA; P = 0.031 for MRE/3'2J/ppC) as determined by Mann-Whitney U test.