Figure 7. A comparison of mosquito numbers and potential virus uptake on defensin expression by western blot analysis. For each viral construct, group A represents 200 larvae and group B represents 300 larvae, fed on 2.7 x 107 virus infected-C6/36 cells. C6/36 cells were inoculated with viruses at a multiplicity of infection of 0.01 for both groups. For the positive control, 0.05 ng purified defensin was used. Larval (4th instar) and pupal samples were collected 5 and 8 days after exposure, respectively, and adult hemolymph was collected 3 days after emergence. The cell control group was exposed to uninfected C6/36 cells.