Figure 6. In vitro Defensin expression in C6/36 cells infected with MRE/3'2J/ppA at MOI of 0.01 (lanes 2 and 4) or MOI of 0.1 (lanes 1 and 3) 72 hours after inoculation. The supernatant of C6/36 cells infected with MRE/3'2J/ppA (lanes 1-4) or MRE/3'2J (lanes 5 and 6) was subjected to western analysis after removing the large molecules in the tissue culture medium using 30 KD molecular weight cutoff ultrafree-MC centrifugal filters (Millipore). 1 µg of protein was loaded in lanes 1-6. Lane 7 shows the hemolymph of an individual Ae. aegypti inoculated with bacteria. Lane 6 is 0.05 ng purified recombinant defensin.