Figure 5. Expression of defensin in the hemolymph of adult Ae. aegypti exposed as larvae to parental MRE, MRE/3'2J/ppA and MRE/3'2J/ppC viruses-infected cells with different multiplicity of infection (MOI). Lane numbers represent different mosquitoes. (A) Western analyses of hemolymph samples of adults collected 3 days after eclosion (cell control: uninfected C6/36 cells, MRE/3'2J, MRE/3'2J/ppA and MRE/3'2J/ppC groups). Individual mosquito carcasses from the lower MOI (0.01) group were titrated (TCID50/ml) after the hemolymph was collected. (B) Hemolymph samples taken 5 days after eclosion from mosquitoes infected with MRE/3'2J/ppA and MRE/3'2J/ppC virus. For the positive control, 0.1 ng purified defensin was used.