Figure 3. Defensin expression in midguts of MRE/3'2J/ppA or ppC virus-infected Ae. aegypti mosquitoes (FITC; patches of defensin peptide are indicated by arrows). Parental MRE/3'2J virus infected mosquitoes showed staining of only the E1 glycoprotein (Texas Red; arrow). Mosquitoes were exposed to virus-infected C6/36 cells as larvae, control group mosquitoes were exposed to uninfected C6/36 cells. Midguts of adult mosquitoes were removed by dissection 3 days after eclosion.