Figure 1. Detection of defensin sequences in recombinant Sindbis viruses by RT-PCR. Viruses were amplified in BHK cells. Cells and parental virus, MRE/3'2J, were used as the negative controls (lane 2 and 3, respectively), and products from MRE/2'3J/ppA (lane 4) and MRE/2'3J/ppC (lane 5) show the defensin gene (arrow). Molecular weight marker is lane 1. No RNA template was added in lane 6.