Table 4 Pedigree and test cross for independent assortment of yellow scale (y), yellow scale plus yellow eye (yes), acetylcholinesterase insensitivity (AceIn) and Heliothis sodium channel protein (codon hscp L1029H).

Member1 AceIn2 Genotype3 Scale color Eye color Hscp4 Grandmother 0.989 AceIn AceIn Green Grey CAT/CAT Grandfather 0.161 AceIn+ AceIn+ Yellow Yellow CTT/CTT Mother 0.582 AceIn AceIn+ Green Grey CTT/CAT Father 0.174 AceIn+ AceIn+ Yellow Yellow CTT/CTT Progeny 17 0.143 AceIn+ AceIn+ Yellow Grey Progeny 16 0.161 AceIn+ AceIn+ Yellow Yellow CTT/CTT Progeny 20 0.164 AceIn+ AceIn+ Yellow Grey Progeny 12 0.463 AceIn AceIn+ Yellow Yellow CTT/CTT Progeny 11 0.561 AceIn AceIn+ Green Grey CTT/CAT Progeny 25 0.569 AceIn AceIn+ Yellow Yellow CTT/CAT Progeny 8 0.586 AceIn AceIn+ Green Grey CTT/CTT Progeny 13 0.601 AceIn AceIn+ Yellow Yellow CTT/CAT Progeny 14 0.692 AceIn AceIn+ Green Grey CTT/CTT Progeny 19 0.793 AceIn AceIn+ Green Grey CTT/CTT Progeny 28 1.154 AceIn AceIn+ Yellow Yellow CTT/CTT Progeny 15 nd AceIn AceIn+ Yellow Grey CTT/CAT

1Members of a pedigree including the maternal grandparents, the parents and 12 offspring. The grandmother was from Dalzell98; the grandfather and the father were from Yyes (strains described in text). 2Acetylcholinesterase insensitivity is represented by the proportion of inhibition when exposed to propoxur for 15 min; i.e. the quotient of the optical density following propoxur exposure corrected for blank divided by the optical density following acetone control exposure corrected for blank. Progeny 15 was not determined (nd) due to a failure of the control. 3Genotype for acetylcholinesterase insensitivity was assigned based upon plots of inhibition as described in the text. Progeny 28 was scored heterozygous based on relative rates of inhibition by propoxur and monocrotophos due to partial failure of the control. Progeny 15 was scored heterozygous based on relative rates of inhibition due to complete failure of the control. 4Genotype for sodium ion channel codon polymorphism for amino acid 1029 as described in the text.